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Enzyme Production from Cassava Peels by Aspergillus awamori Kt-11: The Making of Natural Sweetener from Several tubbers

The use of cassava (Manihot esculenta Crantz) peel for enzyme production has not been widely used. The purpose of this study was to produce complex amylase enzymes from cassava peel by A. awamori KT-11 and apply them in the manufacture of natural sweeteners. Enzyme production is carried out on red and white cassava peel. Media of cassava peel sterilized, inoculated with 1% A. awamori KT-11, incubated for 5 days, then dried at 50°C and mashed. Making sugar is done on cassava flour, sweet potato ( Ipomoea batatas L), taro (Colocasia esculenta) and cocoyam (Xanthosoma sagittifolium) with different concentrations of 10%, 15%, 20%, and 15% and 20% enzyme concentrations. The hydrolysis process is carried out for 3 days at 60°C. The enzyme activity in red cassava peel was 405,006 U/mL and white cassava peel was 321,239 U/ml. The sugar produced in cassava, taro, sweet potato, and Cocoyam was 101.38 mg/mL, 81.18 mg/mL, 55.929 mg/mL, and 42.874 mg/mL, respectively. The results of TLC showed that cassava and taro sugar contain maltose, lactose and glucose, sweet potatoes contained glucose and dextrin and Cocoyam containing fructose. The sweetness level of sugar from cassava, taro, sweet potato and Cocoyam is 14 brix, 12 brix, 9 brix and 9 brix, respectively. ( Ruth Melliawati and Farida Rahman )

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Sumber/Full text : http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/369/pdf

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Diversity And Antimicrobial Activity Of Lichens Associated Actinomycetes In Cibinong Science Centre (CSC) And Cibodas Botanical Garden (CBG) Indonesia

Bioprospecting has developed to all biological taxa including procaryotic. Actinomycetes become interesting procaryotic because of the ability to produce important secondary metabolite for human life. Actinomycetes are known as the largest antibiotic producer that has a broad range habitat. Some research has been done to find new antibiotic from the various habitat of actinomycetes. One of the interesting habitats of actinomycetes which never been explored in Indonesia is lichens... Lichens as the symbiotic structure of alga and fungi areknown as the ecological niche of various kinds of microorganisms including actinomycetes. Cibinong Science Centre (CSC) and Cibodas Botanical Garden (CBG) have various species of trees as the habitat of lichens. These areas are known as one of the research locations to explore the biodiversity of Indonesia. The aims of this research is to study the diversity and antimicrobial potency of actinomycetes isolated from 10 lichen samples with various type of thallus; crustose, fructose and foliose. Lichen samples were grown on the bark of 9 trees species in CSC and CBG. Isolation process used three agar media; HV, YIM6 and YIM711 with cycloheximide and nalidixic acid. Molecular identification based on 16S rRNA gene sequence. Antimicrobial activity was tested to 65 isolates by agar diffusion method to Bacillus subtilis BTCC B.612, Escherichia coli BTCC B.614, Candida albicans BTCC Y.33, Staphylococcus aureus BTCC B.611, Micrococcus luteus BTCC B.552. Isolation process retrieved 125 isolates with the highest number grow on HV agar medium. Based on the sample, the highest number of actinomycetes were isolated from crustose lichen attached on the bark of Averrhoea carambola. A total 69 isolates were identified as the genera Actinoplanes, Amycolatopsis, Angustibacter, Kribbella, Micromonospora, Mycobacterium, and Streptomyces. The screening process showed 24 isolates have antimicrobial activity, with the highest inhibitory activity against Micrococcus luteus BTCC B.552. ( Agustina Eko Susanti , Shanti Ratnakomala , Wibowo Mangunwardoyo ,and Puspita Lisdiyanti)

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Sumber/full text : http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/368/pdf_1

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MAHASISWA UNIVERSITAS AHMAD DAHLAN YOGYAKARTA KUNJUNGI PUSAT PENELITIAN BIOTEKNOLOGI LIPI

 

uad1Cibinong, 28 November 2019Keingintahuan masyarakat akan penelitian dibidang bioteknologi akhir-akhir ini semakin meningkat, hal ini dapat dilihat dengan banyaknya permintaan kunjungan ilmiah ke Pusat Penelitian Bioteknologi LIPI. Pusat Penelitian Bioteknologi LIPI menerima kunjungan dari Universitas Ahmad Dahlan Yogyakarta sebanyak 91 orang. Kegiatan kunjungan ini diterima di Gedung Bioteknologi Peternakan dan dibuka dengan pengenalan dan pemutaran video Profil Pusat Penelitian Bioteknologi-LIPI dan dilanjutkan tur ke laboraorium.

Tujuan dari kunjungan ini adalah selain sebagai kegiatan rutin diluar kampus juga untuk memperdalam ilmu pengetahuan mahasiswa terkait bidang Bioteknologi dengan melihat secara langsung laboratorium yang ada di Pusat Penelitian Bioteknologi-LIPI. Pada kunjungan kali ini para peserta mengunjungi Laboratorium Genetika Molekuler dan Modifikasi Jalur Biosintesis Tanaman, Laboratorium Biokatalis dan Fermentasi dan Laboratorium Biak Sel dan Jaringan Tanaman.

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