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Sequential Adaptation in Mammalian CHO-K1 Cells Producing Human Erythropoietin

 Popi Hadi Wisnuwardhani, Endah Puji Septi Setyani

 Annales Bogorienses, Vol.21 , No.1 (2017) : 15-20

Sequential_Adaptation_in_Mammalian_CHO-K1_Cells_Producing_Human_ErythropoietinThe production of recombinant proteins for clinical applications using mammalian cell technology has become a prevalent system because of its capacity in assembling functional proteins. One of the main problems with CHO-K1 cells is that this cell has to grow in the presence of serum. However, the presence of serum will complicate the downstream step for protein production. Thus, protein produced in media without serum, theoretically, would be easier to purify. Technically, this type of cell can be produced by growing the CHO-K1 cells in serum-free media by using adaptation method in suspension condition. This research showed that through sequential adaptation using conditioned media, the CHO-K1 cell line that produces the human erythropoietin gene (hEPO) was able to grow in suspension culture using serum-free media. Based on Western blot analysis, it showed that the protein (hEPO) was able to be expressed in suspension culture with molecular mass of about 47 kDa.

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Microbial endophite from plant sugar-apple (Annona squamosa l.) as a producer of antimicrobial Staphylococcus aureus and Candida albicans

Ruth Melliawati dan Sunifah

Berita Biologi, 2017, 16 (1): 69-83

Microbial endophite from plant sugar-apple 1Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.

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Pemanfaatan Tandan Kosong Kelapa Sawit untuk Produksi Jamur Tiram (Pleurotus sp.) dan Enzim Ligninase

Firda Dimawarnita, Urip Perwitasari (2017).

Jurnal Mikologi Indonesia, Vol. 1 (2): 100-108

tandanMemanfaatkan secara integratif dan simultan untuk menghasilkan produk bernilai ekonomis tinggi, antara lain jamur tiram (Pleurotus sp.) dan enzim ligninase (Li-P, Mn-P dan lakase). Sistem teknologi secara simultan menghasilkan jamur konsumsi dan enzim ligninolitik (enzim oksidatif) yang dapat dilakukan dengan sistem fermentasi substrat padat TKKS dengan jamur tiram yang merupakan kelompok jamur pelapuk putih/JPP. Tujuan penelitian ini untuk mengembangkan teknologi integratif pengolahan biomassa pertanian (TKKS) dari perkebunan kelapa sawit secara simultan, mengubah TKKS menjadi barang bernilai jual tinggi melalui produksi jamur tiram dan enzim ligninase. Tahap pertama dilakukan pertumbuhan jamur tiram (putih, kuning, dan pink) pada media TKKS. Pertumbuhan jamur konsumsi dilaksanakan di tempat budidaya jamur tiram milik petani di Cianjur. Kemudian setelah pemanenan jamur tiram, seluruh limbah baglog diekstrak dan dianalisis aktivitas enzim ligninolitiknya. Pertumbuhan jamur tiram pink di media TKKS memiliki Biological Efficiency Ratio (BER) sebesar 52,08%, jamur tiram kuning memiliki BER sebesar 41,67%, dan jamur tiram putih memiliki BER 47,92%. Aktivitas enzim ligninolitik yang di ektraksi dari limbah baglog pertumbuhan jamur tiram meliputi aktivitas enzim Lignin peroksidase (Li-P) sebesar 0,57 U/mL, Mangan peroksidase (Mn-P) sebesar 34,22 U/mL, dan lakase sebesar 0,04 U/mL.

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