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Antioxidant Production Of Lactic Acid Bacteria Isolated From Indonesian Traditional Fermented Buffalo Milk (Dadih)

Hasim, AZ Mustopa, N Andrianto, Fatimah, Didah Nur Faridah (2017).

Journal of Pharmacy and Biological Sciences, Vol. 12 (5): 76-82. ISSN 2278-3008.

Microbial endophite from plant sugar-apple 1One of antioxidant sources is foods that contain peptides from enzymatic hydrolysis and/or fermentation by lactic acid bacteria (LAB). The availability of antioxidants in fermented food, particularly milk, is associated with the presence of LAB. Dadih is traditionally made by keeping the fresh raw buffalo milk in a bamboo tube capped with banana leaf and allowing it to ferment spontaneously at room temperature for two days. This research aimed to obtain bacteria from dadih with the highest antioxidants. Isolate Lac 3 and Lac 13 displayed the highest antioxidant activity, with inhibition capacity of 64.2 persen and 63.79 persen respectively against DPPH radical. The result showed that 6 best isolates (Lac 3, Lac 13, DS 6, DS 10, DS 12, and DS 14) were not significantly different. Lac 3 was found to be identical with Lactococcus lactic subsp lactic, while Lac 13 was identical with Lactobacillus plantarum with the percentage of similarity of 98 persen and 96 persen respectively. Lactococcus lactic subsp lactic displayed the highest antioxidant activity at the early stationary phase and at the lowest pH value of culture.

Kata kunci: antioxidant activity, dadih, lactic acid bacteria, milk

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3338&bid=15813

Disusun oleh: Ludya AB/Pustakawan

 

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Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production

Yopi, Nanik Rahmani, Alifah Mafatikhul Jannah, Irfan Pebi Nugraha, Roni Masri Ramadana

Proceedings of the 3rd International Symposium on Applied Chemistry 2017

AIP Conference Proceedings. Vol. 1904, Issue 1: 020022-1–020022-9

https://doi.org/10.1063/1.5011879

Published by AIP Publishing. 978-0-7354-1594-2

Microbial endophite from plant sugar-apple 1Endo- -1, 4-mannanase is the key enzymes for -1,4-linkages within the mannan backbone releasing manno-oligosaccharides (MOS). A marine bacterium of Bacillus subtilis LBF-005 was reported have ability to produce endo-type mannanase. The aims of this research were to compare commercial biomass Locust Bean Gum (LBG) and raw biomass contaning mannan as carbon source for mannanase production from Bacillus subtilis LBF-005, to analyze the optimum condition of mannanase production, and to find out the potential of the mannanase for MOS production. Bacillus subtilis LBF-005 was cultivated in Artificial Sea Water (ASW) medium contain NaCl and various mannan biomass as carbon source for mannanase production. The cells were grown in submerged fermentation. The maximum enzyme activity was obtained with porang potato as a substrate with concentration 1 persen, pH medium 8, and incubation temperature 50°C with an enzyme activity of 37.7 U/mL. The mainly MOS product released by crude mannanase produced by Bacillus subtilis LBF-005 were mannobiose (M2), mannotriose (M3), mannotetraose (M4), and mannopentaose (M5)

Kata kunci: Bacillus subtilis.,cellulase, hemicellulase, marine bacteria, oligosaccharide, mannanase

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3310&bid=15787

Disusun oleh: Ludya AB/Pustakawan

 

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Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.

Yopi, Apridah Cameliawati Djohan, Nanik Rahmani, Alifah Mafatikhul Jannah (2017).

Biofarmasi (Rumphius J NatProd Biochem), 15 (1): 15-20

Microbial endophite from plant sugar-apple 1Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50°C, 5.5;70°C and 8;50°C, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.

Kata kunci: Bacillus sp.,cellulase, hemicellulase, marine bacteria, oligosaccharide

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3309&bid=15784

Disusun oleh: Ludya AB/Pustakawan

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