Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production

Yopi, Nanik Rahmani, Alifah Mafatikhul Jannah, Irfan Pebi Nugraha, Roni Masri Ramadana

Proceedings of the 3rd International Symposium on Applied Chemistry 2017

AIP Conference Proceedings. Vol. 1904, Issue 1: 020022-1–020022-9

https://doi.org/10.1063/1.5011879

Published by AIP Publishing. 978-0-7354-1594-2

Microbial endophite from plant sugar-apple 1Endo- -1, 4-mannanase is the key enzymes for -1,4-linkages within the mannan backbone releasing manno-oligosaccharides (MOS). A marine bacterium of Bacillus subtilis LBF-005 was reported have ability to produce endo-type mannanase. The aims of this research were to compare commercial biomass Locust Bean Gum (LBG) and raw biomass contaning mannan as carbon source for mannanase production from Bacillus subtilis LBF-005, to analyze the optimum condition of mannanase production, and to find out the potential of the mannanase for MOS production. Bacillus subtilis LBF-005 was cultivated in Artificial Sea Water (ASW) medium contain NaCl and various mannan biomass as carbon source for mannanase production. The cells were grown in submerged fermentation. The maximum enzyme activity was obtained with porang potato as a substrate with concentration 1 persen, pH medium 8, and incubation temperature 50°C with an enzyme activity of 37.7 U/mL. The mainly MOS product released by crude mannanase produced by Bacillus subtilis LBF-005 were mannobiose (M2), mannotriose (M3), mannotetraose (M4), and mannopentaose (M5)

Kata kunci: Bacillus subtilis.,cellulase, hemicellulase, marine bacteria, oligosaccharide, mannanase

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3310&bid=15787

Disusun oleh: Ludya AB/Pustakawan

 

Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.

Yopi, Apridah Cameliawati Djohan, Nanik Rahmani, Alifah Mafatikhul Jannah (2017).

Biofarmasi (Rumphius J NatProd Biochem), 15 (1): 15-20

Microbial endophite from plant sugar-apple 1Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50°C, 5.5;70°C and 8;50°C, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.

Kata kunci: Bacillus sp.,cellulase, hemicellulase, marine bacteria, oligosaccharide

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3309&bid=15784

Disusun oleh: Ludya AB/Pustakawan

Sequential Adaptation in Mammalian CHO-K1 Cells Producing Human Erythropoietin

 Popi Hadi Wisnuwardhani, Endah Puji Septi Setyani

 Annales Bogorienses, Vol.21 , No.1 (2017) : 15-20

Sequential_Adaptation_in_Mammalian_CHO-K1_Cells_Producing_Human_ErythropoietinThe production of recombinant proteins for clinical applications using mammalian cell technology has become a prevalent system because of its capacity in assembling functional proteins. One of the main problems with CHO-K1 cells is that this cell has to grow in the presence of serum. However, the presence of serum will complicate the downstream step for protein production. Thus, protein produced in media without serum, theoretically, would be easier to purify. Technically, this type of cell can be produced by growing the CHO-K1 cells in serum-free media by using adaptation method in suspension condition. This research showed that through sequential adaptation using conditioned media, the CHO-K1 cell line that produces the human erythropoietin gene (hEPO) was able to grow in suspension culture using serum-free media. Based on Western blot analysis, it showed that the protein (hEPO) was able to be expressed in suspension culture with molecular mass of about 47 kDa.

Lock full review www.8betting.co.uk 888 Bookmaker

Pusat Penelitian Bioteknologi LIPI