Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1- 0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1- 0031

Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1- 0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1- 0031

Elvi Yetti, Ahmad Thontowi, and Yopi

Annales Bogorienses Vol. 21, No. 2: 38-44

cellDibenzothiophene (DBT) and its derivatives are widely used-model of organic sulfur compounds for the biodegradation process of petroleum oil. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we conducted a follow-up study to determine the optimum conditions of two parameters affecting DBT biodegradation, namely substrate and starter cell concentrations. Three indigenous marine bacteria isolated from Indonesia’s sea environments potentially degrading DBT were examined. The isolates are belong to Mauricauda olearia strain CL-SS4 (99%), Alcanivorax xenomutants strain JC109 (99%), and Stakelama pacifica strain JLT832 (99%). The optimum DBT concentrations act as the growth substrate for all three isolates was 100 ppm, while the optimum cell concentrations for starter inocula was 20 of OD600 nm conversion units.

Keywords: optimization, substrate concentration, starter cell concentration, dibenzothiophene biodegradation, marine bacteria

Katalog: http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/300/pdf

Disusun oleh: Ludya AB/Pustakawan



Genetic Polymorphism Analysis Of 5' Untranslated Region Of Thyroglobulin Gene In Bali Cattle (Bos Javanicus) From Three Different Regions Of Indonesia

pcrThe g.422C>T nucleotide variations in the 5’ untranslated region (5’UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in Bali cattle populations from three different regions as the main resources of Bali cattle in Indonesia.

A total of 200 head of Bali cattle have been performed genotyping on TG5 gene using polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) method and sequence analysis. Results of the study confirmed that TG5 was monomorphic in Bali cattle wherever their origin regions. Moreover, nine candidate SNPs were detected within 5’UTR of TG gene in Bali cattle compared to Genbank reference sequences, although no SNP variations among Bali cattle sample studied. The new other genetic markers within an entire TG gene suggested to be explored and verified for their polymorphisms in Bali cattle. The nine candidate SNPs were also required further verification and validation in a larger sample to be regarded as new SNPs between Bali cattle and Genbank reference sequences.(S. Anwar, A.C. Putra, A.S. Wulandari, P. P. Agung, W.P.B. Putra, S. Said)

Disusun oleh Ahmad S.S/Pustakawan

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=show_detail&id=15931&keywords=

Stabilization of luciferase from Renilla reniformis using random mutations

Megumi Shigehisa, Norie Amaba, Shigeki Arai, Chisato Higashi, Ryo Kawanabe, Ayano Matsunaga, Fina Amreta Laksmi, Masao Tokunaga and Matsujiro Ishibashi

renillaWe expressed luciferase (RLuc) from Renilla reniformis in Escherichia coli. RLuc was purified using a Ni-NTA column and subsequently characterized. It was unstable in acidic solutions and at 30°C. To increase the stability of RLuc, the Rluc gene was randomly mutated using error-prone polymerase chain reaction. E. coli harboring the mutated gene was screened by detecting luminescence on a plate containing the substrate coelenterazine at 34°C. Three mutants, i.e. N264SS287P, N178D and F116LI137V, were obtained. The solubilities and specific activities of these mutants were higher than those of the wild type. Furthermore, the N264SS287P mutant maintained stability at a temperature approximately 5°C higher than that of the wild type, while denaturation of the F116LI137V mutant started at a temperature that was 5°C lower than the wild type, and ended at a temperature that was 7°C higher. We examined the obtained mutations using thermal shift assays and a computer program Coot in this study.

Keywords: Mutation, Stabilization, error-prone PCR, luciferase, Renilla reniformis

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3143&bid=15591

Disusun oleh: Ludya AB/Pustakawan


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