• kakao
  • fgd
  • fgd
  • fgd
  • science sma
  • kunjungan
  • biotek-phapros
  • Kegiatan dengar pendapat antara Kelompok Tani Kopi dan Kakao Desa Genggelang KLU dengan Komisi XI DPR RI, Direktorat Jenderal Perkebunan (Dirjen. Bun.) Kementerian Pertanian Republik Indonesia, Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan (BBPPTP) Medan, Dinas Pertanian dan Perkebunan Provinsi Nusa Tenggara Barat (NTB), Dinas Ketahanan Pangan dan Pertanian KLU, Fakultas Pertanian Universitas Mataram dan Peneliti Pusat Penelitian Bioteknologi-LIPI. Kegiatan ini dilaksanakan di Aula Kelompok Petani Kakao ‘Bunga Mekar’, Dusun Senara, Desa Genggelang, Kecamatan Gangga, KLU, pada 13 September 2017. Pada kegiatan ini, Peneliti Mikrobiologi Terapan Pusat Penelitian Bioteknologi-LIPI, Dr. Fahrurrozi, memaparkan kegiatan kerja sama aplikasi IPTEK fermentasi biji kakao antara Pusat Penelitian Bioteknologi-LIPI dengan Dinas Ketahanan Pangan dan Pertanian, Kabupaten Lombok Utara, NTB.
  • Foto para pembicara pada acara Knowledge Sharing and Discussion dengan Judul "Status Terkini Penelitian dan Pengembangan Bioteknologi Pertanian", di Auditorium Puslit Bioteknologi LIPI-Cibinong, 8 September 2017,sebagai bagian dari rangkaian kegiatan Open House Puslit Bioteknologi LIPI 6-8 September 2017.
  • Foto para pembicara pada Focus Group Discussion (FGD) dengan Judul "Workshop Peran Riset dan Kebijakan Untuk Penguatan Rantai Nilai Ekonomi Ubi Kayu Indonesia", di Auditorium Puslit Bioteknologi LIPI-Cibinong, 7 September 2017,sebagai bagian dari rangkaian kegiatan Open House Puslit Bioteknologi LIPI 6-8 September 2017.
  • Foto bersama Kapuslit Bioteknologi LIPI, Dr. Ir. Bambang Sunarko bersama peserta FGD dengan Judul "Sinergi Konsorsium Kemandirian Bahan Baku Obat : Pengembangan Obat Malaria Dihidroartemisinin", di Auditorium Puslit Bioteknologi LIPI-Cibinong, 6 September 2017,sebagai bagian dari rangkaian kegiatan Open House Puslit Bioteknologi LIPI 6-8 September 2017.
  • Kegiatan Science in Touch, Praktik Isolasi dan Ekstraksi DNA bagi Siswa SMA pada Science Week Fair Kedeputian Bidang IPH LIPI, Cibinong, 4 September 2017. Kegiatan ini bertujuan untuk memperkenalkan bioteknologi bagi para siswa SMA sejak dini dengan cara yang menarik (fun and fearless). Terlihat peneliti bidang biologi molekuler tanaman, Dr. Wahyuni menerangkan apa itu DNA kepada para siswa dengan cara yang populer.
  • Kunjungan Direktur Perencanaan & Pengembangan PT Bio Farma (Persero), drh. Sugeng Raharjo, MM.ke booth pameran Pusat Penelitian Bioteknologi-LIPI dalam bazzar Science Week Fair IPH LIPI, Cibinong, 4 September 2017. Tak lupa pada kesempatan ini Bapak Direktur berfoto bersama dengan Plt. Deputi Bidang Jasa Ilmiah LIPI, Dr. Mego Pinandito M.Eng., Kepala Bidang Pengelolaan dan Diseminasi Hasil Penelitian Puslit Bioteknologi-LIPI, Dr. Ir. Syamsidah Rahmawati, serta para peneliti senior Bidang Peternakan Puslit Bioteknologi-LIPI, Prof. Dr. Endang Tri Margawati dan Dr. Ekayanti Mulyawati Kaiin.
  • Penandatangan Naskah Non Disclosure Agreement (NDA) antara Pusat Penelitian Bioteknologi-LIPI dengan PT Phapros Tbk yang dilaksanakan di Cibinong, 4 September 2017. Pada kesempatan ini Pusat Penelitian Bioteknologi LIPI diwakili oleh Kepala Pusat Penelitian Bioteknologi-LIPI, Dr. Bambang Sunarko dan PT Phapros Tbk diwakili oleh Direktur Utama, Barokah Sri Utami, M.M., Apt., serta disaksikan oleh Plt. Kepala LIPI, Prof. Dr. Bambang Subiyanto, Sekretaris Utama LIPI, Dr. Siti Nuramaliati Prijono, Deputi Ilmu Pengetahuan Hayati LIPI, Prof. Dr. Enny Sudarmonowati, dan Kepala Pusat Inovasi LIPI, Dr. Nurul Taufiqu Rochman, M.Eng. Penandatangan NDA ini merupakan salah satu kegiatan dalam rangkaian acara Science Week Fair Kedeputian Ilmu Pengetahuan Hayati LIPI, 4-9 September 2017.
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Expression of no Affinity Tagged Recombinant Human Interferon Alpha-2a in Methilotropic Yeast Pichia pastoris

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Recombinant human interferon alpha-2a (rhIFNα-2a) has been widely used for clinical therapy as antiviral, anticancer as well as immunomodulator. In this study, The open reading frame (ORF) encoding synthetic hIFNα-2a was constructed to be in framed with N-terminal alpha factor secretion system in methylotropic yeast Pichiapastoris. This research covered construction of no affinity tagged recombinant human interferon alpha-2a, expression and analysis of the molecular weight and identity of the protein. We used pPICZαB plasmid for cloning and expression vector. The confirmed recombinant plasmid containing the correct DNA sequence of hIFNα-2a was linearized by SacI restriction enzyme and then transformed into P. pastorisgenome using electroporation. We screened two multi-copy recombinants in YPDS plates containing zeocin™. Buffered complex medium containing methanol (BMMY) was used for protein expression during 48 hours in the culture condition. The recombinant protein was purified by blue sepharose affinity chromatography. Analyses of protein hIFNα-2a by SDS-PAGE and Western blot confirmed that protein band observed at around 19.2 kDa was recombinant hIFNα-2a. The quantification of purified rhIFNα-2a by using colorimetric binichoninic assay (BCA) informed that the yield was 44 mg/L culture (OD600= 2-3).

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Antiplasmodial activity of methanol extract and pure isolated compound of Calophyllum bicolor P. F. Steven

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Calophyllum bicolor (Clusiacea) is a big tree from Indonesian rain forest in Palangkaraya, Kalimantan. Calophyllum or bintagor is one of many sources of natural bioactive compounds that can be used in the fields of health and pharrmaceuticals. The aim of this research was to explore the ability of methanol extract of Calophyllum bicolor P.F. Steven to inhibit the growth of Plasmodium falciparum. The methanol extract was purified by colomn chromatographyc system, hexane – ethyl acetate was used as solvent with increasing polarity. One pure compound was obtained and was elucidated based on the 1D and 2D-NMR, COSY DEPT, HMBC and HMQC data and the isolated compound was identified as xanthone. Methanol extract showed growth inhibition against parasite P. falciparum with IC50 5.2 ppm and pure compound have maximum inhibition at concentration 0.11 nMol respectively

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Characterization of Phytase, Amylase and Cellulase by Aspergillus niger, Neurospora crassa and Rhizophus oryzae on Sargassum and Rice bran under Solid State Fermentation

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The objective of study was to characterize phytase, amylase, and cellulase on sargassum and rice bran on solid state fermentation using Aspergillus niger, Neurospora crassa and Rhizophus oryzae. Media for solid state fermentations composed of dried sargassum and rice bran. The effect of particle of sargassum, initial moisture content on phytase, amylase, and cellulase were evaluated. Optimum enzyme activity of phytase, amylase and cellulase were obtained after 4 days fermentation at 30 °C, and initial moisture content was adjusted to 60 %. The optimum particle size of dried sargassum attaining the highest enzyme activity was 25 mesh. Best formula for enzymes production was at the ratio of 4:6 (w/w) of Sargassum spinosum (SS) and rice bran (RB) respectively. At this formula highest phytase activity was obtained by A. niger, amylase by N. crassa, and cellulase by R. oryzae. Media composed of sargassum and rice bran can be used for phytase, amylase and cellulase production.

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Pusat Penelitian Bioteknologi LIPI