Print

Transplantasi Sel Punca Bakal Sel Gamet Untuk Mempelajari dan Memanipulasi Spermatogenesis

ekayantiSpermatogenesis adalah proses yang terorganisasi dengan baik meliputi urutan tahap proliferasi dan diferensiasi sel yang menghasilkan spermatozoa dalam jumlah tak terbatas selama kehidupan hewan jantan. Sel punca (stem sel) spermatogonial berpotensi melakukan perbaikan diri sendiri (self-renewal) dan menghasilkan sel-sel anak membentuk spermatozoa. Sel punca spermatogonia merupakan sel yang unik karena merupakan sel yang terdapat pada tubuh dewasa yang dapat membelah dan mengkon tribusikan gennya pada generasi selanjutnya. Assay untuk sel punca spermatogonial dilakukan dengan cara melakukan transplantasi sel-sel gamet dari donor mencit fertil ke testis mencit resipien yang infertil sehingga terjadi proses spermatogenesis sel donor dan produksi sperma pada hewan resipien. Donor mencit jantan dengan gen bacterial b-galactosidase digunakan untuk mengidentifikasi spermatogenesis sel donor pada testis mencit resipien dan menentukan haplotype donor dan dapat memasuki keturunan hewan resipien.

artikel1

Beberapa penelitian telah dilakukan untuk menerapkan teknik transplantasi sel gamet pada hewan tikus. Sel punca spermatogonia mencit dapat dikriopreservasi sebelum ditransplantasikan dan tetap terjadi spermatogenesis pada testis resipien setelah transplantasi. Hal tersebut menandakan adanya potensi genetik dari individu jantan yang immortal, serta dengan teknik analisis yang lebih detail dapat menghitung kolonisasi sel punca donor pada testis resipien. (Ekayanti Mulyawati Kaiin)

Baca artikel lengkap

Print

ROFERON-A: A BIOLOGIC PRODUCT OF HUMAN INTERFERON ALPHA 2A

 Andri Wardiana, Ratih Asmana Ningrum (2015)

roferonHuman interferRoferon Aon alpha 2a (hIFNα2a) is a cytokine regulating immune system that has been used in hepatitis and cancer treatments. It has wide biological potency covering antiviral, antiproliferative and immunomodulative activities. This mini review discusses Roferon-A as a prominent commercial product of recombinant hIFNα2a which is produced in bacterial system, Escherichia coli, as therapeutic protein for several diseases, such as chronic viral Hepatitis B, Hepatitis C, melanoma, hairy cell leukemia and renal cell carcinoma. The discussion focuses on the development process with regard to its manufacturing, preclinical and clinical studies, as well as therapeutic efficacy. In addition, we also discuss biosimilar development of hIFNα2a and its potential future developments in the context of enhancing pharmacokinetic profiles.

 Keywords: interferon alpha 2a, Roferon-A, therapeutic protein and biosimilar.

Katalog : http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3176&bid=15625

 

Print

Removing a Cystein Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein, Even Better

Heni Rachmawati, Adhitya Jessica, Yeyet Cahyati Sumirtaputra, Debbie Sofie Retnoningrum, Amirah Adlia, Ratih Asmana Ningrum (2016).

Removing a CysteinRemoving a Cystein Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein Even Better Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein, Even Better.

Scientia Pharmaceutica. 84: 113–130. ISSN 0036-8709 (Print) ISSN 2218-0532 (Online) ISSN-L 0036-8709

Interferon alpha 2b is the only standard therapeutic protein for hepatitis virus infections. Further study demonstrated that this protein also posseses antitumor activity in several cancerous organs. One main pathway of this antitumor activity is mediated through antiproliferation as well as proapoptotic effects. Previously, we have successfully developed recombinant human interferon alpha 2b (rhIFNá2b) by using a synthetic gene. In addition, two mutein forms of rhIFNá2b were generated to improve the characteristics of this protein. Two point mutations showed better pharmacokinetic profiles than one point mutation as well as the native form. In the present study, this mutein form was studied for ist antitumor effect in vitro using HepG2 cells. As a comparison, the native form as well as a commercial rIFNá2b were used. Several parameters were investigated including the MTT assay, cell viability test, cell cycle using flow cytometric analysis, and the genes and protein expressions involved in cell growth. The latest was observed to study the mechanism of rhIFNá2b. There was no significant difference in the MTT assay and cell viability after cells were treated with both forms of rhIFNá2b. However, the mutein rhIFNá2b tended to show better proapoptotic activity reflected by flow cytometric data, protein expression of pSTAT1, and DNA expression of caspase 3.

Keywords: Interferon alpha 2b, Antiproliferation, Apoptosis, p21k1, p27, Caspase 3, pSTAT1, Flow cytometri, Mutein

Sumber: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3022&bid=15484

Lock full review www.8betting.co.uk 888 Bookmaker

Pusat Penelitian Bioteknologi LIPI