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Low cost and comprehensive pork detection in processed food products with a different food matrix

Low cost and comprehensive pork detection in processed food products with a different food matrix

Fenny Aulia Sugiana, Henni Widyowati, M. Ali Warisman, Suryani, and Desriani (2018)

Indonesian Journal of Biotechnology, 23 (1): 21-27.ISSN 0853-8654

 Low cost and comprehensive pork detection in processed food products with a different food matrixThe adulteration of processed beef-based meat products with pork is a sensitive issue in Indonesia. Therefore a simple, low cost, and accurate method is required for the detection of pork, so as to protect consumers from accidental consumption of adulterated meat. In this study, we developed a detection method for the low cost identification of pork in processed meat products. We used the cost-efficient Taq DNA polymerase, DreamTaq Green PCR master mix (2x), and duplex PCR method to recognize pork simultaneously with 18S rRNA detection. A positive control containing a pork gene inserted into pGEM®-T easy was prepared, along with a negative control. The results of the duplex PCR were used to assess its specificity, detection limit, and its ability to recognize pork in processed meat products with a different food matrix. 18S rRNA detection was for confirming DNA integrity of DNA extracted from the processed food, while the positive control confirmed that the reagents were working well and the negative control confirmed a non-contamination problem. Following this, the duplex PCR was opধmized and the optimum concentration primer for duplex PCR detection was found to be 3 µM for pork and 0.2 µM for 18S rRNA. As liħle as 3.125 ng of the DNA template could be used to detect whether a sample contained pork. Of the nine commercial processed meat products tested, five were found to contain pork while four halal products showed no signs of pork. It can be concluded that duplex PCR is a simple, fast, sensitive, specific, and low cost method of detecting pork in processed meat products.

Keywords: 18S rRNA, duplex PCR, low cost, pork, processed meat

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3480&bid=16089

Disusun oleh: Ludya AB/Pustakawan

 

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Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

Nahdaturrugaisiyah, Azamris, Bugi Ratno Budiarto, I Made Artika, Desriani (2018)

Pak. J. Biotechnol., Vol. 15 (3): 735-742.

 Tandem Recombinant Plasmid Construction as Positive ControlPIK3CA H1047R mutation is found in breast cancer in high frequency and its detection could be applied as prognosis and predictive factor for trastuzumab therapy. qPCR is one of the simplest and robust method for PIK3CA H1047R detection. Provision of positive control for PIK3CA H1047R detection based on qPCR will support data analysis efficiently and avoid false negative result. In this research, we constructed a tandem recombinant plasmid (pGEM-tandPIK3CA) as positive control for Tm Shift SYBR Green I qPCR-based of PIK3CA H1047R detection system by ligating wild-type and PIK3CA H1047R fragments tandemly into pGEM-T Easy. The tandem plasmid was confirmed by restriction, DNA sequencing and qPCR. As a result, pGEMtandPIK3CA has been successfully constructed and confirmed. Statistical analysis shows high repeatability and reproducibility with % CV of <25%. The main advantage of this tandem positive control is its ability to serve as positive control for both wild-type PIK3CA and PIK3CA H1047R simultaneously, therefore improving the efficiency of the detection system.

Keywords: Breast cancer, PIK3CA H1047R, qPCR, positive control.

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3475&bid=16075

Disusun oleh: Ludya AB/Pustakawan

 

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Antimicrobial Activity of Selenium Nanoparticles Synthesized by Actinomycetes Isolated from Lombok Island Soil Samples

Antimicrobial Activity of Selenium Nanoparticles Synthesized by Actinomycetes Isolated from Lombok Island Soil Samples

Shanti Ratnakomala, Nurul Fitri Sari, Fahrurrozi and Puspita Lisdiyanti (2018)

Jurnal Kimia Terapan Indonesia. Vol. 20 (1): 8-15. e-ISSN: 2527–7669.

 Antimicrobial Activity of Selenium NanoparticlesA total of 98 actinomycetes were isolated from the soil and litter samples collected from the cacao and coffee plantation in Lombok Island, West Nusa Tenggara of Indonesia. These isolates were screened for their antimicrobial activity. Among 98 isolated strains, only 24 isolates showed antimicrobial activity against test microorganisms, 20.4% were active against Bacillus subtilis BTCC B-612, 14.3% against Staphylococcus aureus BTCC B-611, and 5.1% against Escherichia coli BTCC B-609. Of these 24 isolates, 3 were found to be able to grow in medium containing 3 mM Selenium oxide and the culture were changed color to red. Two of the best strains, L-155 and L-156, were selected for assessing production of Selenium nanoparticles. Bioreduction of selenium nanoparticles was confirmed by UVvisible spectrophotometer that showed the peak between 300 and 320 nm. Biosynthesized selenium nanoparticle from isolate actinomycetes L-155 and L-156 were found to have a broad spectrum of activity against the tested microorganisms Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Micrococcus luteus, and Candida albicans. The study shows the rapid and eco-friendly synthesis of selenium nanoparticles from soil actinomycetes. Most of these active isolates showed to possessed antibacterial property.

Keywords: Lombok Island, actinomycetes, selenium nanoparticles, antimicrobial activity

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3472&bid=16045

Disusun oleh: Ludya AB/Pustakawan

 

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Pertumbuhan Kultur Tunas Taka Pada Media MS yang Mengandung Sitokinin dan Manitol untuk Konservasi In Vitro

Pertumbuhan Kultur Tunas Taka Pada Media MS yang Mengandung Sitokinin dan Manitol untuk Konservasi In Vitro

Betalini Widhi Hapsari, Andri Fadillah Martin, Tri Muji Ermayanti (2018)

Prosiding Seminar Nasional Pertanian dan Tanaman Herbal Berkelanjutan di Indonesia, Hal. 35-43. Universitas Muhammadiyah Jakarta. e-ISSN: 2615-2320.

 taccaTanaman taka (Tacca leontopetaloides (L.) Kuntze) merupakan jenis tanaman yang tumbuh terbatas di beberapa daerah pantai di Indonesia. Tanaman ini merupakan tanaman minor sehingga perlu dikonservasi. Umbi taka berpotensi sebagai sumber karbohidrat. Tujuan dari penelitian ini adalah untuk mengetahui pertumbuhan kultur tunas taka pada media yang mengandung sitokinin BAP atau kinetin yang dikombinasikan dengan manitol untuk konservasi secara in vitro. Penelitian dilakukan di Laboratorium Biak Sel dan Jaringan Tanaman Pusat Penelitian Bioteknologi LIPI Cibinong Science Center pada bulan Juni 2016 sampai Maret 2017. Setelah dikonservasi selama 24 minggu, planlet diaklimatisasi. Tunas taka ditumbuhkan selama 24 minggu pada media MS yang mengandung 0, 0.5 ppm BAP dan 0.5 ppm kinetin dikombinasikan dengan manitol 0%, 2%, 4%, dan 6%. Kultur diinkubasi pada ruang bersuhu 25 oC. Percobaan menggunakan Rancangan Acak Lengkap (RAL) dengan 3 ulangan, data dianalisis dengan ANOVA dilanjutkan dengan uji Duncan Multiple Range Test (DMRT). Variabel pengamatan yang diamati setiap minggu selama 24 minggu adalah tinggi tanaman, jumlah daun dan jumlah akar serta daya hidup setelah aklimatisasi. Hasil penelitian menunjukkan bahwa Media MS tanpa sitokinin dan tanpa manitol (kontrol) dan media MS yang mengandung 0.5 ppm kinetin tanpa manitol menunjukkan pertumbuhan terbaik pada semua variabel pengamatan. Taka yang berasal dari media MS tanpa manitol, MS + 2% manitol, MS + 0.5 ppm BAP tanpa manitol, MS + 0.5 ppm BAP + 2% manitol, MS + 0.5 ppm kinetin tanpa manitol, dan MS + 0.5 ppm kinetin + 2% dan 4%manitol mampu tumbuh di rumah kaca.

Keywords: Tacca leontopetaloides, in vitro, manitol, sitokinin BAP, kinetin, konservasi.

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3471&bid=16044

Disusun oleh: Ludya AB/Pustakawan

 

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Pengaruh Modifikasi KH2PO4, NH4NO3 dan Sukrosa terhadap Pertumbuhan Tunas serta Pembentukan Umbi Mikro Taka (Tacca leontopetaloides) secara In vitro

Pengaruh Modifikasi KH2PO4, NH4NO3 dan Sukrosa terhadap Pertumbuhan Tunas serta Pembentukan Umbi Mikro Taka (Tacca leontopetaloides) secara In vitro

Rudiyanto, Betalini Widhi Hapsari & Tri Muji Ermayanti (2018).

Jurnal Biologi Indonesia, Vol. 14 (1): 11-21. ISSN 0854-4425; E-ISSN 2338-834X.

 taccaPolynesian arrowroot (Tacca leontopetaloides (L.) Kuntze), which is one of the bulbous herbaceous plants, have high nutritional value. Modification of macro nutrients by reducing nitrogen content and increasing phosphorus on the medium gave affects on shoot growth and initiated micro tuber formation on in vitro cultures. The aim of this research was to determine the effect of modified macro nutrients in combination with the increase in sucrose concentrations on shoot growth and micro tuber formation of T. leontopetaloides. The experimental design was factorial completely randomized design. The factors tested were modifications of MS macro nutrients that were. M1 (170 mg/l KH2PO4 and 1650 mg/l NH4NO3; normal, control treatment); M2 (340 mg/l KH2PO4 and 825 mg/l NH4NO3); and M3 (680 mg/l KH2PO4 and 412.5 mg/l NH4NO3 in combination with 30 (S1) (control treatment), 40 (S2), 50 (S3) and 60 g/l of sucrose (S4). The variables tested were shoot height, number of leaves, number of roots and number of micro tuber which were observed weekly at 0-8 weeks after culturing. The results showed that the modification of macro nutrient in combination with sucrose concentration had significant effect on shoot height, number of leaves and number of roots but not significant on the number of tubers. The highest shoots were found in M1S3 treatment, the highest number of leaves was in M1S1 and M1S3 treatment and the highest number of roots was in M1S4 treatment. The number of tubers not significantly different between the treatments tested.

Keywords: in vitro, KH2PO4, microtuber, NH4NO3, sucrose, Tacca leontopetaloides.

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3468&bid=15997

Disusun oleh: Ludya AB/Pustakawan

 

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