Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production

Yopi, Nanik Rahmani, Alifah Mafatikhul Jannah, Irfan Pebi Nugraha, Roni Masri Ramadana

Proceedings of the 3rd International Symposium on Applied Chemistry 2017

AIP Conference Proceedings. Vol. 1904, Issue 1: 020022-1–020022-9

Published by AIP Publishing. 978-0-7354-1594-2

Microbial endophite from plant sugar-apple 1Endo- -1, 4-mannanase is the key enzymes for -1,4-linkages within the mannan backbone releasing manno-oligosaccharides (MOS). A marine bacterium of Bacillus subtilis LBF-005 was reported have ability to produce endo-type mannanase. The aims of this research were to compare commercial biomass Locust Bean Gum (LBG) and raw biomass contaning mannan as carbon source for mannanase production from Bacillus subtilis LBF-005, to analyze the optimum condition of mannanase production, and to find out the potential of the mannanase for MOS production. Bacillus subtilis LBF-005 was cultivated in Artificial Sea Water (ASW) medium contain NaCl and various mannan biomass as carbon source for mannanase production. The cells were grown in submerged fermentation. The maximum enzyme activity was obtained with porang potato as a substrate with concentration 1 persen, pH medium 8, and incubation temperature 50°C with an enzyme activity of 37.7 U/mL. The mainly MOS product released by crude mannanase produced by Bacillus subtilis LBF-005 were mannobiose (M2), mannotriose (M3), mannotetraose (M4), and mannopentaose (M5)

Kata kunci: Bacillus subtilis.,cellulase, hemicellulase, marine bacteria, oligosaccharide, mannanase


Disusun oleh: Ludya AB/Pustakawan


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