Biotek

Rapat Pembahasan Kerjasama LIPI dengan PT. ASTRA

            Pusat Penelitian Bioteknologi–LIPI, pada tanggal 19 Juni 2019, bertempat di Ruang Rapat Gedung Administrasi, mengadakan rapat pembahasan perpanjangan kerja sama LIPI dengan PT. ASTRA. Kegiatan yang dipimpin Kapuslit Bioteknologi-LIPI, Puspita Lisdiyanti ini dihadiri oleh, Kepala Biro Umum LIPI, perwakilan Biro Kerja Sama, Hukum dan Humas-LIPI, para peneliti di lingkungan Deputi Bidang Ilmu Pengetahuan hayati dan perwakilan PT. ASTRA.

            Kegiatan rapat kerja sama ini membahas kelanjutan dan perpanjangan kerja sama yang telah dirintis dua tahun sebelumnya. Tujuan makro dari perpanjangan kerja sama ini untuk melanjutkan misi melestarikan tumbuhan buah langka nusantara dan pengembangan plasma nutfah buah lokal yang sudah terlaksana selama dua tahun sebelumnya dan akan dilanjutkan selama dua tahun mendatang.

            Dari kerja sama ini kedua belah pihak mengharapkan terdapat area pelestarian buah langka dan pengembangan plasma nutfah buah lokal nusantara yang dapat menjadi tempat penelitian, pendidikan, wisata (eco-eduwisata), konservasi buah langka dan laboratorium plasma nutfah buahlokal. Selain itu, diharapkan kerja sama yang notabene terbangun dari program Coorporate Social Responsibility (CSR) PT. Astra International, Tbk. ini dapat mendukung pelestarian lingkungan hidup dan kemajuan ilmu pengetahuan dan teknologi di bidang konservasi (Yovita/ed:Ang)

Kecepatan Regenerasi Kalus Somatik Embriogenik Terung Pada Beberapa Media Maturasi

Kecepatan Regenerasi Kalus Somatik Embriogenik Terung Pada Beberapa Media Maturasi

Hartati, Hanifah Agani, N. Sri Hartati, Enny Sudarmonowati (2018)

Jurnal ILMU DASAR, Vol.19 (2): 125-134. ISSN-2442-5613

terungRalstonia solanacearum is one of the most important pathogen that causes bacterial wilt disease in eggplant and inhibits eggplant production. Improvement of eggplant varieties resistant to bacterial wilt can be accomplished through genetic manipulation. Regeneration of in vitro plants isone of the important tools to supports plant improvementthrough biotechnology. This study was aimed to determine the rate of eggplant regeneration in various maturation media, and to find the best medium for eggplant regeneration based on maturation rate and the number of cotyledon produced. We used resistant eggplant (accession 032)as the material to produce somatic embryogenic.There were 7 types of regeneration media used in this research. MS medium was supplemented with a certain concentration of plant growt regulators , such as: 1 mg / L + BAP 1 mg / L, NAA 4mg / L, TDZ 0.005 mg / L, TDZ 0.001 mg / L, CuSO4 2mM + BAP 1 mg / L, CuSO4 2mM + BAP 2 mg / L and Kinetin 1 mg / L + CuSO4 2mM. Three clumps of callus per plate with three replications were transferred to MS suplemented medium. The parameters observed were the color of callus before and after they were transfered to regeneration medium, the day of formation of globular, heart-shaped, tubular and cotyledonary phase, and the number of cotyledons formed. The results obtained showed the somatic embryogenic color of the 032 genotype was white with friable structure before being transferred to regeneration medium and was turned to yellowish white after being transferred to the regeneration medium. On the day sixth, friable embryogenic somatic of eggplant was developed into nodule on medium MS + NAA 4 mg / L, MS + CuSO4 2mM + BAP medium 1 mg / L, and MS + CuSO4 2mM + BAP 2 mg / L. Somatic embryogenic callus of accession 032 were able to pass complete globular, heart-shaped, tubular and cotyledonary phase. The most responsive medium for somatic embryogenic callus regeneration, based on the days of the callus phases formation and the number of early-phase cotyledons obtained, were MS medium suplemented with CuSO4 2mM + BAP,and CuSO4 2 mM + BAP 2 mg / L.

Keywords: eggplant, Ralstonia solanacearum, regeneration, cotyledonary, clump, BAP

Katalog: http://perpus.biotek.lipi.go.id/perpus/index.php?p=fstream&fid=3486&bid=16092

Disusun oleh: Ludya AB/Pustakawan

Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances

Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances

Ariani Hatmanti, Puspita Lisdiyanti, Jaka Widada, and Subagus Wahyuono (2018)

Squalen Bull. of Mar. and Fish. Postharvest and Biotech, Vol. 13 (2): 45-56. ISSN: 2089-5690 e-ISSN: 2406-9272

 antimicrobialA study on isolation of actinomycetes from sediments of Makassar Strait have been conducted with regard to research project called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014. The objectives of this research were to screen antimicrobial activity of 36 actinomycetes from sediments of Makassar Strait, characterized the potential isolates, and determined the metabolites produced by the selected isolate. The antimicrobial screening was conducted using agar diffusion method, while characterization of the best five of actinomycetes were using APIZYM Kit, Scanning Electron Microscope, and FTIR. Five isolates retrieved from this research had ability to inhibit the growth of four microbial testing: Escherichia coli, Bacillus subtilis, Staphyllococcus aureus and Candida albicans. The highest capability was shown by the MACMK-43 isolate that had 16S rRNA gene sequence similarity of 97.85% to Streptomyces violacens. The result shows that, the active fraction contained of 4-amino-5-cyano-6-(4’methoxyphenyl)-1-methyl-2.3 dihydropyrrolo [2,3-B] pyridine, which is commercially used for bactericide and antihistamine.

Keywords: antimicrobes, actinomycetes, Streptomyces violascens, deepsea sediment.

Katalog: http://perpus.biotek.lipi.go.id/perpus/index.php?p=fstream&fid=3485&bid=16091

Disusun oleh: Ludya AB/Pustakawan

Low cost and comprehensive pork detection in processed food products with a different food matrix

Low cost and comprehensive pork detection in processed food products with a different food matrix

Fenny Aulia Sugiana, Henni Widyowati, M. Ali Warisman, Suryani, and Desriani (2018)

Indonesian Journal of Biotechnology, 23 (1): 21-27.ISSN 0853-8654

 Low cost and comprehensive pork detection in processed food products with a different food matrixThe adulteration of processed beef-based meat products with pork is a sensitive issue in Indonesia. Therefore a simple, low cost, and accurate method is required for the detection of pork, so as to protect consumers from accidental consumption of adulterated meat. In this study, we developed a detection method for the low cost identification of pork in processed meat products. We used the cost-efficient Taq DNA polymerase, DreamTaq Green PCR master mix (2x), and duplex PCR method to recognize pork simultaneously with 18S rRNA detection. A positive control containing a pork gene inserted into pGEM®-T easy was prepared, along with a negative control. The results of the duplex PCR were used to assess its specificity, detection limit, and its ability to recognize pork in processed meat products with a different food matrix. 18S rRNA detection was for confirming DNA integrity of DNA extracted from the processed food, while the positive control confirmed that the reagents were working well and the negative control confirmed a non-contamination problem. Following this, the duplex PCR was opধmized and the optimum concentration primer for duplex PCR detection was found to be 3 µM for pork and 0.2 µM for 18S rRNA. As liħle as 3.125 ng of the DNA template could be used to detect whether a sample contained pork. Of the nine commercial processed meat products tested, five were found to contain pork while four halal products showed no signs of pork. It can be concluded that duplex PCR is a simple, fast, sensitive, specific, and low cost method of detecting pork in processed meat products.

Keywords: 18S rRNA, duplex PCR, low cost, pork, processed meat

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3480&bid=16089

Disusun oleh: Ludya AB/Pustakawan

 

Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

Tandem Recombinant Plasmid Construction as Positive Control for PIK3CA H1047R Detection Based on SYBR Green I qPCR

Nahdaturrugaisiyah, Azamris, Bugi Ratno Budiarto, I Made Artika, Desriani (2018)

Pak. J. Biotechnol., Vol. 15 (3): 735-742.

 Tandem Recombinant Plasmid Construction as Positive ControlPIK3CA H1047R mutation is found in breast cancer in high frequency and its detection could be applied as prognosis and predictive factor for trastuzumab therapy. qPCR is one of the simplest and robust method for PIK3CA H1047R detection. Provision of positive control for PIK3CA H1047R detection based on qPCR will support data analysis efficiently and avoid false negative result. In this research, we constructed a tandem recombinant plasmid (pGEM-tandPIK3CA) as positive control for Tm Shift SYBR Green I qPCR-based of PIK3CA H1047R detection system by ligating wild-type and PIK3CA H1047R fragments tandemly into pGEM-T Easy. The tandem plasmid was confirmed by restriction, DNA sequencing and qPCR. As a result, pGEMtandPIK3CA has been successfully constructed and confirmed. Statistical analysis shows high repeatability and reproducibility with % CV of <25%. The main advantage of this tandem positive control is its ability to serve as positive control for both wild-type PIK3CA and PIK3CA H1047R simultaneously, therefore improving the efficiency of the detection system.

Keywords: Breast cancer, PIK3CA H1047R, qPCR, positive control.

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3475&bid=16075

Disusun oleh: Ludya AB/Pustakawan

 

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Pusat Penelitian Bioteknologi LIPI