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Removing a Cystein Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein, Even Better

Heni Rachmawati, Adhitya Jessica, Yeyet Cahyati Sumirtaputra, Debbie Sofie Retnoningrum, Amirah Adlia, Ratih Asmana Ningrum (2016).

Removing a CysteinRemoving a Cystein Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein Even Better Group On Interferon Alpha 2b at Position 2 and 99 does Not Diminish Antitumor Activity of the Protein, Even Better.

Scientia Pharmaceutica. 84: 113–130. ISSN 0036-8709 (Print) ISSN 2218-0532 (Online) ISSN-L 0036-8709

Interferon alpha 2b is the only standard therapeutic protein for hepatitis virus infections. Further study demonstrated that this protein also posseses antitumor activity in several cancerous organs. One main pathway of this antitumor activity is mediated through antiproliferation as well as proapoptotic effects. Previously, we have successfully developed recombinant human interferon alpha 2b (rhIFNá2b) by using a synthetic gene. In addition, two mutein forms of rhIFNá2b were generated to improve the characteristics of this protein. Two point mutations showed better pharmacokinetic profiles than one point mutation as well as the native form. In the present study, this mutein form was studied for ist antitumor effect in vitro using HepG2 cells. As a comparison, the native form as well as a commercial rIFNá2b were used. Several parameters were investigated including the MTT assay, cell viability test, cell cycle using flow cytometric analysis, and the genes and protein expressions involved in cell growth. The latest was observed to study the mechanism of rhIFNá2b. There was no significant difference in the MTT assay and cell viability after cells were treated with both forms of rhIFNá2b. However, the mutein rhIFNá2b tended to show better proapoptotic activity reflected by flow cytometric data, protein expression of pSTAT1, and DNA expression of caspase 3.

Keywords: Interferon alpha 2b, Antiproliferation, Apoptosis, p21k1, p27, Caspase 3, pSTAT1, Flow cytometri, Mutein

Sumber: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3022&bid=15484

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Glucoamylase Production by Aspergillus awamori KT-11 In Solid-State Fermentation Using Cassava Peel as Substrate

Urip Perwitasari, Nuryati, Ruth Melliawati, and Yopi (2017). Glucoamylase Production by Aspergillus awamori KT-11 In Solid-State Fermentation Using Cassava Peel as Substrate. Annales Bogorienses, Vol. 21 (1): 21-28. ISSN 0517-8452

Glucoamylase Production by Aspergillus awamori KT-11 In Solid-State Fermentation Using Cassava Peel as Substrate

Cassava has long been known as one of the main staple food in Indonesia. Whereas the cassava peel contains starch of approximately 72%, it is still underrated as a carbohydrate source for fermentation. The utilization of cassava peel as a substrate in solid state fermentation potentially replaces rice as a carbon source leading to more cost-effective production. This study aims at producing glucoamylase by means of solid state fermentation using Aspergillus awamori KT-11 and cassava peel as substrate. The study demonstrated that medium composition and drying technique affected the production of glucoamylase. The highest glucoamylase activities were identified when cassava peel and mineral media was used in fermentation, compared to only cassava peel; the combination of cassava peel, mineral, and rice bran; rice media or a mixture of rice, mineral and rice bran. Freeze-dried glucoamylase, furthermore, exhibited higher specific activity in contrast to the oven-dried one, with 452 U/mL and 365 U/mL, respectively. In conclusion, cassava peel plus mineral is a better substrate for glucosamine production by A. awamori KT-11 in solid state fermentation. Besides, powdered glucoamylase had been demonstrated to be capable of hydrolyzing starch-based biomass.

Keywords: glucoamylase, cassava peel, A. awamori KT-11, solid state fermentation

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Endophytic Fungi Associated With Turmeric (Curcuma longa L.) Can Inhibit Histamine-Forming Bacteria in Fish

Eris Septiana, Nampiah Sukarno, Sukarno, Partomuan Simanjuntak (2017). Endophytic Fungi Associated With Turmeric (Curcuma longa L.) Can Inhibit Histamine-Forming Bacteria in Fish. HAYATI Journal of Biosciences, (2017) 24: 46-52. ISSN: 1978-3019. DOI: 10.1016/j.hjb.2017.05.004

Turmeric (Curcuma longa L.) is a medicinal plant that is commonly used as spice and preservative. Many types of endophytic fungi have been reported as being associated with medicinal plants and able to synthesize secondary metabolites. In this study, endophytic fungi were isolated from all plant parts of turmeric plants. Identification of the endophytic fungi was done using morphological characteristics and sequencing of the internal transcribed spacer (ITS) region of ribosomal DNA. The dual culture method was used for screening antibacterial activity of the endophytic fungi against Morganella morganii, a common histamine-producing bacteria. The disc diffusion method was used to test the ability of water fractions of selected endophytic fungi to inhibit M. morganii growth. Two-dimensional thin layer chromatography was used to determine the fungal extract inhibition activity on histamine formation. In total, 11 endophytic fungi were successfully isolated and identified as Arthrobotrys foliicola, Cochliobolus kusanoi, Daldinia eschscholzii, Fusarium oxysporum, Fusarium proliferatum, Fusarium solani, Fusarium verticillioides, Phanerochaete chrysosporium, and Phaeosphaeria ammophilae. Five isolates showed inhibition activity against M. morganii in the dual culture tests. Based on the disc diffusion assay, A. foliicola and F. verticillioides inhibited the growth of M. morganii as a histamine-producing bacteria, and inhibiting histamine formation in fish. The best effects in inhibiting growth of the histamine-producing bacteria and histamine formation inhibition in fish were produced with F. verticillioides water fraction at 0 °C incubation.

Keywords: antimicrobial activity, medicinal plant, Morganella morganii, phylogenetic analysis, ribosomal DNA

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Manipulasi Sel Somatik dan Transgenesis Tanaman Talas

MANIPULASI SEL SOMATIK DAN TRANSGENESIS TANAMAN TALAS

Andri Fadillah Martin, N. Sri Hartati, Aida Wulansari, Siti Noorohmah, Pramesti Dwi Aryaningrum dan Witjaksono

Seminar Nasional Hasil Penelitian Unggulan Bidang Pangan Nabati. Bogor, 25 September 2014. 75-90 hal.

Kelangkaan pangan telah MANIPULASI SEL SOMATIK DAN TRANSGENESIS TANAMAN TALASmenjadi ancaman setiap negara, semenjak meningkatnya pertumbuhan penduduk dunia, sehingga dunia akan menghadapi ancaman karena ketidakmampuan mengimbangi pertumbuhan penduduk dengan penyediaan pangan yang memadai. Dengan demikian, diperlukan suatu usaha untuk mengurangi ketergantungan pangan pokok dari komoditi beras. Salah satu alternatif yang perlu dikembangkan adalah pengembangan tanaman umbi-umbian yang kini mulai ditinggalkan oleh masyarakat. Komoditas umbi dari sumber daya lokal yang potensial untuk dikembangkan adalah talas (Colocasia esculenta L. Schott.). Talas merupakan tanaman herba yang digunakan sebagai makanan pokok di daerah Pasifik. Di Indonesia, talas memiliki keanekaragaman genetik yang besar sehingga potensi perakitan galur unggul talas ini terbuka lebar baik secara konvensional maupun bioteknologi. Tujuan dari penelitian ini adalah mengembangkan protokol untuk mendapatkan varietas unggul melalui poliploidisasi, fusi protoplas dan transgenesis tanaman talas. Penelitian manipulasi sel somatik talas terdiri dari induksi mutasi dengan sinar gamma, induksi poliploid dengan oryzalin dan fusi protoplas. Percobaan fusi protoplas dimulai dari induksi kalus meremah yang dilanjutkan dengan pengembangan protokol isolasi dan fusi protoplas. Telah diperoleh tunas-tunas hasil radiasi sinar Gamma yang dapat digunakan untuk seleksi lebih lanjut. Induksi poliploidisasi talas berhasil dilakukan dengan didapatkannya kultur talas poliploid. Protokol fusi protoplas dikembangkan melalui fusi antara protoplas dua jenis talas berbeda yang diisolasi dari daun dan kalus. Penelitian transgenesis talas meliputi pengembangan metoda transformasi genetik menggunakan faktor transkripsi 35S-OSHOX4 dan analisis molekuler planlet hasil transformasi. Hasil yang diperoleh dari penelitian rekayasa genetika talas untuk ketahanan terhadap kekeringan adalah protokol transfromasi genetik gen 35S-OSHOX4 mengggunakan Agrobacterium tumefaciens dan planlet putatif transgenik. Plantlet-plantlet in vitro talas yang diperoleh dari penelitian manipulasi somatik maupun transformasi genetik diharapkan dapat diuji lebih lanjut untuk pengembangan galur baru talas unggul.

Kata kunci: Talas, induksi mutasi, induksi poliploid, transformasi genetik, Agrobacterium tumefaciens.

Sumber: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3155&bid=15601

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Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061

Karakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061

Puspasari NoerwKarakterisasi Biodegradasi Senyawa Poliaromatik Dibenzothiophene Oleh Bakteri Laut Novosphingobium mathurense LBF-1-0061an Tanjung, Elvi Yetti, Ahmad Thontowi, Agung Suprihadi, Susiana Purwantisari & Yopi

Jurnal Biologi Indonesia 12 (2): 257-264 (2016)

Dibenzotiofen merupakan kelompok hidrokarbon polisiklik aromatik yang memiliki kandungan sulfur. Senyawa ini memiliki sifat toksik, mutagenik dan sulit terdegradasi di lingkungan. Dari uji skrining diketahui bahwa isolat LBF-1-0061 termasuk bakteri yang mampu mendegradasi dibenzotiofen. Penelitian ini bertujuan untuk mengetahui potensi isolat bakteri laut LBF-1-0061 dalam mendegradasi dibenzotiofen melalui uji skrining, analisis residu dibenzotiofen dengan GC/M dan untuk mengidentifikasi isolat tersebut secara molekular. Isolat LBF-1-0061 mampu tumbuh dalam dibenzotiofen hingga konsentrasi 100 ppm dalam 14 hari inkubasi. Hasil penelitian menunjukkan bahwa degradasi dibenzotiofen terjadi hingga 37,5%. Identifikasi sebagian gen isolat LBF-1-0061 dilakukan berdasarkan gen 16s rRNA. Hasil analisis menunjukkan bahwa isolat tersebut memiliki tingkat kemiripan sebesar 99% dengan Novosphingobium mathurense strain SM117.

Kata Kunci : Bakteri laut, Biodegradasi, Dibenzotiofen, Hidrokarbon polisiklik aromatik

http://perpus.biotek.lipi.go.id/perpus/index.php?p=fstream&fid=3152&bid=15600

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