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Optimization Of Enzymatic Hydrolysis Of Fish Protein Hydrolysate (FPH) Processing From Waste Of Catfish Fillet Production

enzymeAn experiment to determine the optimum condition of fish protein hydrolysate (FPH) processing has been conducted to produce FPH with high protein content. The raw material was catfish (Pangasius sp.) waste from catfish fillet production. Fat content, sensory (color, odor and flavor), total number of bacteria (TPC) and E. coli were analyzed as supporting parameters. The experiment was initiated with preliminary experiment to determine the optimum temperature (temp variation: 50oC, 55oC, 60oC, and 65oC) and concentration of commercial papain enzyme (conc variation: 4%, 6%, and 8%) in FPH hydrolysis, while the main experiment was to determine the optimum time of hydrolysis (time variation: 6, 12, 24, 36, and 48 hours), and pH (pH variation: 5 and 7). Results showed that the optimum temperature in preliminary experiment was 60oC and optimum concentration of enzyme was 4%, whereas the main experiment resulted the optimum hydrolysis time of 48 hours and optimum pH of 5. This condition produced FPH with protein content of 1.21% wet weight basis (wb) or 39.03% dry weight basis (db) and fat content of 0.16% wb (5.16% db). The sensory analysis of the end product revealed that from max score of 5, the scores of color, odor and flavor of the FPH were 3.50, 2.17 and 2.29, respectively. The product had TPC of 1.1x 107CFU/g with no E. coli infection. (Bagus Sediadi Bandol Utomo, Theresia Dwi Suryanigrum, Herbert R Harianto)

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Desalination Of Chitoologosaccaharides Using Gel Filtration And Ultrafiltration

maldi-tofChitooligosaccharide (COS) , which is a derivative product from chitosan, has recently been used as a functional food because it has antimicrobial, antifungal, and antitumor properties. The salt content in chitooligosaccharide is one of the main problems in application as functional food or pharmaceutical medicine. The aim of this study was to remove salt from COS with two desalting techniques and determine the variation of COSs in the product. The desalting technique used were dialysis with 10kD Molecular Weight Cut Off (MWCO) and gel filtration chromatography HiPrep 26/10 desalting with G-25 Superfine Sephadex as stationary phase in the column. In order to detect the presence of COS, Thin Layer Chromatography (TLC) method was used, followed by Matrix-Assisted Laser Desorption-Ionization Time-of-Flight Mass Spectrometry (MALDI–TOF–MS) to detect low concentration of COS. Qualitative and quantitative analysis of salt presence were identified using silver nitrate and Volhard method respectively. Ash content was measured using gravimetric method. Results showed those dialysis and gel filtration chromatographies were successfully remove the most of the salt from COS with efficiency of desalting up to 100%. However, the best desalting technique was gel filtration chromatography HiPrep 26/10 which has more complete COS with various degrees of polymerization present in the result. (Pujoyuwono Martosuyono, Asri Pratitis, Alexander Prasetya and Elisabeth Kartika Prabawati )

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OPTIMIZATION OF BACTERIOCIN PRODUCTION BY Lactococcus Lactis Ssp. Lactis CN1.10a ORIGIN FROM RUSIPS

optbacPrevious study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a isolated from rusip, a traditional Bangkanese fermented fish product.The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2L). Followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity on that scale, and also stability of bacteriocin during storage.The results showed that the optimum temperature and pH for the growth of producer cell were 28°C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH2PO4, 0,2% NaCl and 0,02% MgSO4.7H20) in addition of sucrose as carbohydrate source. Based on the growth curve performedon CM medium with KH2PO4, the L. Lactis ssp lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was 8000 AU/mlat24th hour.Bacteriocin was relatively stable when stored at -20°C for 1month with a relative activity of 69,4%. (Ninoek Indriati, Arifah Kusmarwati and Irma Hermana )

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Immunostimulatory Effect Of Fatty Acid From Star Fish (Acanthaster Planci) On Lymphocyte Proliferation In-Vitro

lymphocyteThe aims of this study were to investigate lymphocyte proliferation activity and to identify chemical constituents of active fractions of star fish Acanthaster planci. A. planci was collected from Ternate Island, North Moluccas, extracted with distilled methanol and water, partitioned with gradient chloroform-hexane-methanol-water and fractionated in column chromatography using silica gel and hexane-ethyl acetate-methanol. The active compound had been purified by Thin Layer Chromatography (TLC) and identified by Gas Chromatography-Mass Spectrometry (GC-MS). The lymphocyte proliferation activity was measured based on % Stimulation Index (SI) from sample absorbency and control absorbency. The result showed that the 3 fractions of hexane fraction exhibited lymphocyte proliferation activity. Fraction 1 was able to increase lymphocyte proliferation at 48 hours and 72 hours by 55% (80 µl/ml) and 88% (160 µl/ml) increase, and fraction 2 had 77% (160 µl/ml) and 86% (640 µl/ml) increase. Meanwhile, fraction 3 had 75% (640 µl/ml) and 89% (640 µl/ml) increase. Metabolite analysis of active fraction using GC-MS yielded a number of chemical constituents that was dominated by fatty acid. The study concluded that star fish A. planci from Ternate Island has a potential source of immunostimulator. (M. Janib Achmad1, Alim Isnansetyo, Noer Kasanah, Ustadi, and Kamiso)

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Tinea Imbrikata

hiperpigmentasiTinea imbrikata adalah dermatofitosis kronis yang disebabkan oleh Trychophyton concentricum dengan gambaran morfologis khas, berupa papulo-skuamosa yang tersusun dalam lingkaran-lingkaran konsentris, sehingga tampak seperti atap genting. Dilaporkan satu kasus tinea imbrikata pada wanita usia 47 tahun. Pada pemeriksaan fisik, didapatkan lesi kulit dengan distribusi generalisata hampir di seluruh bagian tubuh, kecuali wajah, telapak tangan dan kaki, berupa skuama halus yang tersusun konsentris. Pada pemeriksaan mikroskopis dengan pewarnaan KOH 10% didapatkan hifa panjang, spora, dan epitel. (Reyshiani Johan)

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Manifestasi Klinis Sindrom Behcet

behcetSindrom Behcet adalah proses inflamasi multisistemik yang tidak diketahui etiologinya, manifestasi klinis berupa ulkus oral rekuren, ulkus genital, lesi kulit, lesi mata, dan berbagai sistem organ lain. Kasus wanita 21 tahun mengeluh luka-luka kecil yang nyeri di rongga mulut sejak tiga minggu, hilang timbul hampir setiap bulan sejak lima tahun. Luka juga terdapat di kemaluan, hilang timbul sejak empat tahun dan berulang tiga hingga empat kali setiap tahun. Kedua mata merah dan berair, sejak satu tahun. Pada pemeriksaan kedua mata tampak injeksi konjungtiva dan tidak ada penurunan visus. Pada rongga mulut didapatkan ulserasi aftosa multipel berdiameter 0,6 cm. Pada vulva terdapat ulkus menggaung dengan tepi meninggi berukuran 3 cm x 1,5 cm x 0,5 cm. Pasien didiagnosis sebagai sindrom Behcet berdasarkan International Classification Criteria of Behcet’s Disease atau menggunakan skoring Revised International Criteria for Behcet Disease (ICBD). Pengobatan kortikosteroid dan antibiotik oral atau topikal. Tujuan terapi adalah mempercepat penyembuhan dan mencegah remisi. Luka membaik selama tiga minggu pengobatan. (Sukmawati Tansil Tan, Listyani Gunawan, Gabriela Reginata)

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Neovaskularisasi Koroid Miopia

funduskopiNeovaskularisasi Koroid (NVK) merupakan salah satu komplikasi miopia dan miopia maligna yang mengancam penglihatan. Risiko NVK meningkat seiring dengan peningkatan angka kejadian miopia. Sampai saat ini belum ada definisi standar NVK pada miopia atau miopia maligna. Pasien NVKm dapat mengeluh gangguan visus, metamorfopsia, dan skotoma. Fluorescein angiography dan optical tomography dapat digunakan untuk diagnosis NVKm. Tatalaksana NVKm terus berkembang, anti-VEGF memperbaiki, mengendalikan penyakit, dan diharapkan dapat memperbaiki tajam penglihatan. (Elvira,Victor Nugroho Wijaya)

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