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Proses Menua, Stres Oksidatif, dan Peran Antioksidan

proses menuaPenuaan ditandai dengan penurunan progresif integritas fisiologis yang memicu gangguan fungsi, yang disebabkan oleh radikal bebas sebagai hasil stres oksidatif ditambah modifikasi genetik dan lingkungan. Intervensi yang membatasi atau menghambat reaksi radikal bebas akan menurunkan laju perubahan akibat penuaan, sehingga diharapkan dapat menurunkan tingkat penuaan dan patogenesis penyakit. Antioksidan merupakan molekul yang mampu menstabilkan atau menonaktifkan radikal bebas sebelum menyerang sel, juga dapat menghambat ataupun menunda oksidasi suatu substrat. Antioksidan memiliki fungsi preventif dan protektif terhadap penyakit terkait usia seperti penyakit kardiovaskuler, kanker, kelainan neurodegeneratif, dan berbagai kondisi kronik lainnya. (Marta Lisnawati Zalukhu, Agustinus Rudolf Phyma, Rizaldy Taslim Pinzon)

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Perbandingan Akurasi Diagnostik Kadar Procalcitonin dan C-Reactive Protein pada Pasien Appendisitis Anak di RSUP H. Adam Malik Medan dan RSUD Dr. Pirngadi Medan

procalcitoninLatar Belakang: Diagnosis dari appendisitis akut masih merupakan suatu tantangan, terutama pada kelompok usia anak. Oleh karena itu, mutlak diperlukan suatu alat diagnostik yang sensitif dan spesifik. Tujuan: membandingkan nilai akurasi antara procalcitonin (PCT) dan C-reactive protein (CRP) dalam mendiagnosis appendisitis anak. Metode: penelitian potong lintang dengan analisis deskriptif dan analitik terhadap 31 pasien usia dibawah 18 tahun yang datang ke IGD RSUP H. Adam Malik Medan dengan gejala appendisitis dan telah menjalani appendektomi dari bulan Desember 2014 hingga Juli 2015. Seluruh sampel darah diambil dan dikirim ke laboratorium untuk pengukuran nilai PCT dan CRP preoperatif. Dilakukan appendektomi dan pemeriksaan histopatologi pasca-operasi. ROC nilai sensitivitas, spesifisitas, prediksi positif, dan prediksi negatif dianalisis menggunakan kurva untuk masing-masing pemeriksaan laboratorium dan kombinasi keduanya.

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Pertemuan Forum Pustakawan LIPI Wilayah Bogor Cibinong dan Cibodas (FORKAWAN BCC) tanggal 21 Februari 2018, di Ruang Petemuan Gedung InaCC, Puslit Biologi LIPI, Cibinong

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FORKAWAN BCC adalah Forum komunikasi yang mewadahi dan beranggotakan pustakawan LIPI yang berasal dari Pusat penelitian Biologi, Pusat Penelitian Bioteknologi, Pusat Penelitian Limnologi, Pusbindiklat Peneliti, PKT Kebun Raya Bogor, BKT Kebun Raya Cibodas, dan beberapa satuan kerja lainnya. Tujuan pembentukan Forkawan BCC (Bogor, Cibinong, Cibodas) untuk bersama-sama mengembangkan potensi diri untuk membentuk sebuah forum komunikasi sebagai sarana dalam rangka mengkaji keilmuan dan pengembangan karir jabatan fungsional pustakawan melalui pemahaman, penerapan dan pengembangan teori, teknik, serta teknologi yang berguna untuk mendukung fungsi dan tugas perpustakaan di lembaga induk.

Salah satu agenda kegiatan yang dilakukan adalah pertemuan rutin 3 bulan sekali yang membahas perkembangan TIK di era revolusi industri 4.0, dimana teknologi informasi telah menjadi basis dalam kehidupan manusia. Segala hal menjadi tanpa batas (borderless) dengan penggunaan daya komputasi dan data yang tidak terbatas (unlimited), karena dipengaruhi oleh perkembangan internet dan teknologi digital yang masif sebagai tulang punggung pergerakan dan konektivitas manusia dan mesin. Era ini juga akan mendisrupsi berbagai aktivitas manusia, termasuk di dalamnya bidang ilmu pengetahuan dan teknologi (iptek) serta pendidikan tinggi.

Untuk mengawali tahun 2018 pertemuan FORKAWAN BCC di adakan pada tanggal 21 Februari 2018 di Ruang Pertemuan Gedung InaCC - Puslit Biologi LIPI.

Tujuan dari pertemuan ini:
1. Knowledge sharing
2. Berkontribusi aktif memajukan lembaga
3. Meningkatkan kompetensi pustakwan
4. Silaturahim

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The search for antimicrobial compounds from marine invertebrate-associated bacteria In Indonesian coral reef ecosystems: Presentasi RUTI

reef invetebrataIndonesia is the world's largest archipelagic country with approximately 17,508 islands containing valuable coastal and marine resources such as coral reefs. Marine organisms, in particular marine invertebrates from coral reef ecosystems have become sources of great interest to natural product chemistry, since they produce metabolites with different biological activities.

 

Marine invertebrates, which are plentiful in the Indo-Pacific regions including Indonesia, are rich in secondary metabolites and are becoming targets of continuing search for bioactive compounds. However, the problem of supply has hampered the development of these secondary metabolites produced by marine invertebrates. On the contrary, the metabolites from microorganisms is a rapidly growing field, due, at least in part, to the suspicion that a number of metabolites obtained from algae and invertebrates may be produced by associated microorganisms.

 

The project is aimed at isolating the microorganisms associated with reefs invertebrates from geographically different sites; screening of secondary metabolite producing­microorganisms by using biological and molecular approach, PCR-NRPS (Non­ribosomal Peptide synthetases) and PKS (Polyketide synthases) to increase the efficiency of screening; identifying prospective microorganisms; cloning and sequencing of the gene fragments of PKS and NRPS; bioassay-guided purification as well as dissemination of project results.

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Development of Citrus Rootstock Resistance to Fungal Diseases by Gene Technology : Presentasi RUTI 2007

citrus infectedOne of the main constrains on citrus production is diseases of the root and stem- caused by Phytophthora and Diplodia. A number of fungal endophytes isolated from citrus root and stem are known to be antagonistic to those pathogenic fungi. These endophytes produce chitinase (Chn) and glucanase (Glu) enzymes which lyse the phytopathogenic fungal cell-wall during the antagonistic action, and release cell-wall fragments that act as elicitors of defence reactions such as accumulation of phytoalexins.

It has been envisaged that chitinase and glucanase derived from antagonist strains of fungi can be used as heterologous genes in crop plants for enhancing their resistance against phytopathogenic fungi. Although in some cases, treatment with glucanase and chitinasecan inhibit fungal growth in vitro, more often combinations of the two enzymes are required for antifungal activity.

This research has been conducted to insert and have expressed in citrus rootstocks, the ßGlu and Chn genes isolated from fungal endophytes of citrus roots and stem,to enhance their resistance to root and stem infecting fungi.

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Developing Blast Durable Resistance By Using The Wild Rice Species Oryza rufipogonpogon : Presentasi RUTI IV: 2005-2007

padi blastOryza rufipogon is one of wild rice species that may be used as a source of gene (s) in rice varietal improvement program. Backcross (BC5) and double haploid populations had been developed from a cross between IR64 and O. rufipogon in order to exploit the useful genes for blast disease resistance. These two populations were used to map blast resistant genes and to develop durably resistant varieties. This study has been conducted in collaboration with CIRAD with the following objectives: (1) to identify resistance genes to blast, Pir4(t), originated from O. rufipogon and Pir7(t) originated from IR64, on chromosome 2, and (2) to evaluate the durable resistance of double haploid lines generated from BC2F3 of IR64 x O. rufipogon to blast. Result of the previous study indicated that Pir (4&7) was mapped in the position between two Simple Sequence Repeat (SSR) markers RM263-RM250, with the distance of 28.5 cM (Utami et al, 2005). Fine map is to be established to detect the exact position of the target genes. For this purpose, two lines BC5 population, namely 317-25-1-6 and 317-25-1-3 were selected as lines with the needed level of introgression for fine mapping and target gene identification. In order to complement and to enhance candidate gene analysis at the target position the insilico analysis was applied at the target fragment region peak between RM263-RM250 markers, which resulted in consecutive order 25 putative resistance genes in this fragment region. These putative genes were further analyzed by sequencing the bases of the selected genes above to design specific primer on the basis of TIGR gene genome browser. These designed primers are Single Nucleotide Polymorphism (SNP) that has high polymorphism levels to be used to enhance fine mapping development activities.

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