Oryza rufipogon is one of wild rice species that may be used as a source of gene (s) in rice varietal improvement program. Backcross (BC5) and double haploid populations had been developed from a cross between IR64 and O. rufipogon in order to exploit the useful genes for blast disease resistance. These two populations were used to map blast resistant genes and to develop durably resistant varieties. This study has been conducted in collaboration with CIRAD with the following objectives: (1) to identify resistance genes to blast, Pir4(t), originated from O. rufipogon and Pir7(t) originated from IR64, on chromosome 2, and (2) to evaluate the durable resistance of double haploid lines generated from BC2F3 of IR64 x O. rufipogon to blast. Result of the previous study indicated that Pir (4&7) was mapped in the position between two Simple Sequence Repeat (SSR) markers RM263-RM250, with the distance of 28.5 cM (Utami et al, 2005). Fine map is to be established to detect the exact position of the target genes. For this purpose, two lines BC5 population, namely 317-25-1-6 and 317-25-1-3 were selected as lines with the needed level of introgression for fine mapping and target gene identification. In order to complement and to enhance candidate gene analysis at the target position the insilico analysis was applied at the target fragment region peak between RM263-RM250 markers, which resulted in consecutive order 25 putative resistance genes in this fragment region. These putative genes were further analyzed by sequencing the bases of the selected genes above to design specific primer on the basis of TIGR gene genome browser. These designed primers are Single Nucleotide Polymorphism (SNP) that has high polymorphism levels to be used to enhance fine mapping development activities.
Cibinong -, Ketika pada suatu waktu seorang pimpinan bertanya tentang sekumpulan berkas pada stafnya : “Berkas apa tuh?Tolong serahkan saja ke perpustakaan,” katanya pada staf tersebut. Tunggu dulu! Benarkah berkas tersebut merupakan berkas yang memang merupakan tugas dan wewenang perpustakan untuk mengolah, mengelola dan mendokumentasikannya? Sebagai petugas yang menerima berkas pertama kali, semestinya dia memilah berkas tersebut apakah akan diserahkan ke perpustakaan atau ke pusat arsip.