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Pectinase Production and Clarification Treatments of Apple (Malus Domestica) Juice

Pectinase Production and Clarification Treatments of Apple (Malus Domestica) Juice

Cocok Ana Maryani B, Fahrurrozi, Anja Meryandini

Annales Bogorienses Vol. 21, No. 2: 63-68

Pectinases are a group of enzymes that break down pectin, a polysaccharide that is found in plant cell walls. Today, the application of pectinolytic enzymes plays an important role in food technology for the maceration of fruits and vegetables, including for the extraction and clarification of juice. This research aimed to produce pectinase enzyme for clarifying apple juice. A microbial culture was selected from cocoa bean fermentation samples and identified as Bacillus sp.. Citrus pectin (1%) as the carbon source and peptone (0.1%) as the nitrogen source was found as the best component for pectinase production. The optimum condition of pectinase activity was observed at pH 5 and temperature 40 °C. Enzyme stability studies were performed by incubating crude extract at and the crude enzyme at 40 °C and the percentage activity decrease after one hour storage. Apple juice was treated with the enzyme at different concentrations (0%, 0.5%, 1%, 2%, 4%). Apple juice clarification was evaluated for its percent clarity and viscosity. The result showed that enzyme treatment at 4% in apple juice promoted juice clarification and decreased pH value. In conclusion, the quality of apple juice can be improved by enzymatic treatment using pectinase.

Keywords: Bacillus, clarification, apple juice, pectinase

Katalog: http://http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/311/pdf

Disusun oleh: Ludya AB/Pustakawan

 

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ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique

ILE655VAL Genotyping Study of HER2 - Positive Breast Cancer of Patients from Padang - Indonesia With High Resolution Melting Technique

Dwi Wulandari, Azamris Azamris, Isna Nurhayati, M Ali Warisman, Bugi Ratno Budiarto, Desriani Desriani

Annales Bogorienses Vol. 21, No. 2: 69-75

her2Trastuzumab has proven to be a great improvement in the treatment of HER2+ breast cancer patients, but it is associated with relevant adverse cardiac events and significantly elevated cost of treatment. One of the risk factor for cardiotoxicity due to trastuzumab is the I655V HER2 polymorphism (GTC> ATC mutation) in which the allele mutant (Ile/Val or Val/Val) has a higher risk than the wild type (Ile/Ile). The detection of specific alleles is very important for therapeutic decision-making. In this study, our group has developed a high resolution melting (HRM) with EvaGreen dye method to discriminate specific allele of I655V HER2 (wild type, heterozygote mutant or homozygote mutant) in 66 frozen section samples of HER2+ breast cancer patients. Our study revealed that the wild type is the most prevalent allele (77,27%), whereas heterozygous mutation is significantly present in this research (21.21%) and around 1.52% of samples were detected as minor allele. Only one sample was detected as a minor allele (Val/Val) and may have relatively low abundance in the population. This method has been compared to Sanger sequencing and shows 100% of validity.

Keywords: Breast cancer, HER2, I655V, HRM, allele

Katalog: http://http://jurnal.biotek.lipi.go.id/index.php/annales/article/view/310/pdf

Disusun oleh: Ludya AB/Pustakawan

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Cegah Penyebaran Covid-19, LIPI Desinfeksi Kawasan CSC-BG Cibinong

Cibinong, Humas LIPI. Dalam upaya pencegahan penyebaran wabah Covid-19 yang semakin meluas, LIPI melakukan desinfeksi pada fasilitas publik dan semua area satuan kerja di Kawasan Cibinong Science Center-Botanical Garden (CSC-BG) pada Jumat (3/20), yang meliputi P2 Biomaterial, P2 Biologi, P2 Bioteknologi, P2 Limnologi, PPII, Gedung A Kawasan, Gedung Widya Selaras, dan Rusunawa LIPI. “Sesuai arahan Kepala LIPI, untuk upaya pencegahan penyebaran Covid-19 kami melakukan agenda penyemprotan desinfektan di seluruh Kawasan CSC-BG LIPI. Karena area cukup luas yaitu ada sekitar 9 gedung, jadi ada 5 tim yang akan bertugas”, jelas Ahmad Fathoni, Ketua PME Kedeputian Bidang IPH LIPI. 

Fathoni menjelaskan, ada sekitar 100 liter lebih cairan desinfektan yang akan digunakan untuk di seluruh kawasan. Desinfeksi diprioritaskan pada ruangan yang dipakai oleh banyak sivitas LIPI, yaitu ruang rapat, fasilitas gedung dan bagian luar laboratorium (koridor), dan tempat ibadah. “Untuk yang paling krusial seperti Gedung PPII yang masih ada beberapa pertemuan yang diadakan, kedepannya akan dilakukan penyemprotan desinfektan setiap hari, jadi setelah ada pertemuan seperti rapat bisa langsung disterilkan”, ungkapnya.

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