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Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production

Yopi, Nanik Rahmani, Alifah Mafatikhul Jannah, Irfan Pebi Nugraha, Roni Masri Ramadana

Proceedings of the 3rd International Symposium on Applied Chemistry 2017

AIP Conference Proceedings. Vol. 1904, Issue 1: 020022-1–020022-9

https://doi.org/10.1063/1.5011879

Published by AIP Publishing. 978-0-7354-1594-2

Microbial endophite from plant sugar-apple 1Endo- -1, 4-mannanase is the key enzymes for -1,4-linkages within the mannan backbone releasing manno-oligosaccharides (MOS). A marine bacterium of Bacillus subtilis LBF-005 was reported have ability to produce endo-type mannanase. The aims of this research were to compare commercial biomass Locust Bean Gum (LBG) and raw biomass contaning mannan as carbon source for mannanase production from Bacillus subtilis LBF-005, to analyze the optimum condition of mannanase production, and to find out the potential of the mannanase for MOS production. Bacillus subtilis LBF-005 was cultivated in Artificial Sea Water (ASW) medium contain NaCl and various mannan biomass as carbon source for mannanase production. The cells were grown in submerged fermentation. The maximum enzyme activity was obtained with porang potato as a substrate with concentration 1 persen, pH medium 8, and incubation temperature 50°C with an enzyme activity of 37.7 U/mL. The mainly MOS product released by crude mannanase produced by Bacillus subtilis LBF-005 were mannobiose (M2), mannotriose (M3), mannotetraose (M4), and mannopentaose (M5)

Kata kunci: Bacillus subtilis.,cellulase, hemicellulase, marine bacteria, oligosaccharide, mannanase

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3310&bid=15787

Disusun oleh: Ludya AB/Pustakawan

 

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Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.

Yopi, Apridah Cameliawati Djohan, Nanik Rahmani, Alifah Mafatikhul Jannah (2017).

Biofarmasi (Rumphius J NatProd Biochem), 15 (1): 15-20

Microbial endophite from plant sugar-apple 1Isolation, identification, and characterization of mannanase, xylanase and cellulase producing indigenous marine bacteria have been conducted from total 20 isolates. Based on 16S rDNA sequence analysis, three potential isolates are obtained and identified as Bacillus subtilis (M8), Bacillus tequilensis (X4) and Bacillus cereus (C9). The potential strains M8, X4 and C9 can produce mannanase, xylanase and cellulase activities such as 9.5 U/mL; 0.36U/mL;0.56U/mL with optimum pH and temperature 6.0;50°C, 5.5;70°C and 8;50°C, respectively. Based on the TLC analysis, mannanase from M8 and xylanase from X4 has potential to hydrolyzed mannan and xylan for producing oligosaccharides with size around tri-hexasaccharides as a main product.

Kata kunci: Bacillus sp.,cellulase, hemicellulase, marine bacteria, oligosaccharide

Katalog: http://perpus.biotek.lipi.go.id/index.php?p=fstream&fid=3309&bid=15784

Disusun oleh: Ludya AB/Pustakawan

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Kebun Plasma Nutfah Puslit Bioteknologi LIPI dikunjungi SD Plus Cerdas Alam Rizkia

Cibinong, Humas LIPI. Pusat Penelitian Bioteknologi Lembaga Ilmu Pengetahuan Indonesia (LIPI), pada (Kamis 10/10) menerima kunjungan SD Plus Cerdas Alam Rizkia Bogor, Rombongan yang berjumlah 54 orang terdiri dari 45 orang siswa dan 9 orang guru pendamping. Rombongan berkunjung ke kebun plasma nutfah dan mendapatkan informasi tentang kegiatan yang dilakukan  dirumah kasa model hidroponik sayuran dan model budidaya sayuran organik. “Sayuran organik dibudidayakan secara alami tanpa tambahan bahan kimia. Media tanam yang menggunakan pupuk, pemillihan bibit, proses tanam, pemeliharaan dan pembasmian hama tidak menggunakan bahan kimia yang notabene dapat mengganggu kesehatan” ungkap, Heru Wibowo yang merupakan salah seorang  teknisi di Laboratorium Agronomi untuk Evaluasi Produk Bioteknologi Pusat Penelitian Bioteknologi LIPI.

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