Optimization of Substrate and Starter Cell Concentrations for Dibenzothiopene Biodegradation by Indigeneous Marine Bacteria Mauricauda olearia LBF-1- 0009, Alcanivorax xenomutants LBF-1-0018, and Stakelama pacifica LBF-1- 0031
Elvi Yetti, Ahmad Thontowi, and Yopi
Annales Bogorienses Vol. 21, No. 2: 38-44
Dibenzothiophene (DBT) and its derivatives are widely used-model of organic sulfur compounds for the biodegradation process of petroleum oil. The abilities of microorganisms to degrade pollutants are significantly influenced by various factors such as microbial species, nutrients and environmental parameters. In this research, we conducted a follow-up study to determine the optimum conditions of two parameters affecting DBT biodegradation, namely substrate and starter cell concentrations. Three indigenous marine bacteria isolated from Indonesia’s sea environments potentially degrading DBT were examined. The isolates are belong to Mauricauda olearia strain CL-SS4 (99%), Alcanivorax xenomutants strain JC109 (99%), and Stakelama pacifica strain JLT832 (99%). The optimum DBT concentrations act as the growth substrate for all three isolates was 100 ppm, while the optimum cell concentrations for starter inocula was 20 of OD600 nm conversion units.
Keywords: optimization, substrate concentration, starter cell concentration, dibenzothiophene biodegradation, marine bacteria
Disusun oleh: Ludya AB/Pustakawan
The g.422C>T nucleotide variations in the 5’ untranslated region (5’UTR) of TG gene (called as TG5) has been reported to be associated with level in intramuscular fat (IMF) content or marbling in beef cattle. The objective of this study was to confirm genetic polymorphism of TG5 gene in Bali cattle populations from three different regions as the main resources of Bali cattle in Indonesia.
A total of 200 head of Bali cattle have been performed genotyping on TG5 gene using polymerase chain reaction-restriction fragment lenght polymorphism (PCR-RFLP) method and sequence analysis. Results of the study confirmed that TG5 was monomorphic in Bali cattle wherever their origin regions. Moreover, nine candidate SNPs were detected within 5’UTR of TG gene in Bali cattle compared to Genbank reference sequences, although no SNP variations among Bali cattle sample studied. The new other genetic markers within an entire TG gene suggested to be explored and verified for their polymorphisms in Bali cattle. The nine candidate SNPs were also required further verification and validation in a larger sample to be regarded as new SNPs between Bali cattle and Genbank reference sequences.(S. Anwar, A.C. Putra, A.S. Wulandari, P. P. Agung, W.P.B. Putra, S. Said)
Disusun oleh Ahmad S.S/Pustakawan
- Stabilization of luciferase from Renilla reniformis using random mutations
- Aktivitas Antibakteri Aktinomisetes Laut Dari Pulau Enggano [Antibacterial activity of marine actinomycetes from Enggano Island]
- Production of Manooligomannan from Palm Kernel Cake by Mannanase Produced from Streptomyces Cyaenus
- Body Weight Estimation of Bali Cattle in Banyumulek Techno Park, West Nusa Tenggara using Several Morphometric Parameters
- Antioxidant Production Of Lactic Acid Bacteria Isolated From Indonesian Traditional Fermented Buffalo Milk (Dadih)
- Production of Mannanase from Bacillus Subtilis LBF-005 and Its Potential for Manno-oligosaccharides Production
- Isolation and characterization of mannanase, xylanase, and cellulase from marine bacteria Bacillus sp.
- Sequential Adaptation in Mammalian CHO-K1 Cells Producing Human Erythropoietin
- Microbial endophite from plant sugar-apple (Annona squamosa l.) as a producer of antimicrobial Staphylococcus aureus and Candida albicans
- Pemanfaatan Tandan Kosong Kelapa Sawit untuk Produksi Jamur Tiram (Pleurotus sp.) dan Enzim Ligninase