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Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances

Potency of Actinomycetes from Deepsea Sediment of Makassar Strait for Producing Antimicrobial Substances

Ariani Hatmanti, Puspita Lisdiyanti, Jaka Widada, and Subagus Wahyuono (2018)

Squalen Bull. of Mar. and Fish. Postharvest and Biotech, Vol. 13 (2): 45-56. ISSN: 2089-5690 e-ISSN: 2406-9272

 antimicrobialA study on isolation of actinomycetes from sediments of Makassar Strait have been conducted with regard to research project called Widya Nusantara Exploration (EWIN) in May-June 2013 and November 2014. The objectives of this research were to screen antimicrobial activity of 36 actinomycetes from sediments of Makassar Strait, characterized the potential isolates, and determined the metabolites produced by the selected isolate. The antimicrobial screening was conducted using agar diffusion method, while characterization of the best five of actinomycetes were using APIZYM Kit, Scanning Electron Microscope, and FTIR. Five isolates retrieved from this research had ability to inhibit the growth of four microbial testing: Escherichia coli, Bacillus subtilis, Staphyllococcus aureus and Candida albicans. The highest capability was shown by the MACMK-43 isolate that had 16S rRNA gene sequence similarity of 97.85% to Streptomyces violacens. The result shows that, the active fraction contained of 4-amino-5-cyano-6-(4’methoxyphenyl)-1-methyl-2.3 dihydropyrrolo [2,3-B] pyridine, which is commercially used for bactericide and antihistamine.

Keywords: antimicrobes, actinomycetes, Streptomyces violascens, deepsea sediment.

Katalog: http://perpus.biotek.lipi.go.id/perpus/index.php?p=fstream&fid=3485&bid=16091

Disusun oleh: Ludya AB/Pustakawan

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Kecepatan Regenerasi Kalus Somatik Embriogenik Terung Pada Beberapa Media Maturasi

Kecepatan Regenerasi Kalus Somatik Embriogenik Terung Pada Beberapa Media Maturasi

Hartati, Hanifah Agani, N. Sri Hartati, Enny Sudarmonowati (2018)

Jurnal ILMU DASAR, Vol.19 (2): 125-134. ISSN-2442-5613

terungRalstonia solanacearum is one of the most important pathogen that causes bacterial wilt disease in eggplant and inhibits eggplant production. Improvement of eggplant varieties resistant to bacterial wilt can be accomplished through genetic manipulation. Regeneration of in vitro plants isone of the important tools to supports plant improvementthrough biotechnology. This study was aimed to determine the rate of eggplant regeneration in various maturation media, and to find the best medium for eggplant regeneration based on maturation rate and the number of cotyledon produced. We used resistant eggplant (accession 032)as the material to produce somatic embryogenic.There were 7 types of regeneration media used in this research. MS medium was supplemented with a certain concentration of plant growt regulators , such as: 1 mg / L + BAP 1 mg / L, NAA 4mg / L, TDZ 0.005 mg / L, TDZ 0.001 mg / L, CuSO4 2mM + BAP 1 mg / L, CuSO4 2mM + BAP 2 mg / L and Kinetin 1 mg / L + CuSO4 2mM. Three clumps of callus per plate with three replications were transferred to MS suplemented medium. The parameters observed were the color of callus before and after they were transfered to regeneration medium, the day of formation of globular, heart-shaped, tubular and cotyledonary phase, and the number of cotyledons formed. The results obtained showed the somatic embryogenic color of the 032 genotype was white with friable structure before being transferred to regeneration medium and was turned to yellowish white after being transferred to the regeneration medium. On the day sixth, friable embryogenic somatic of eggplant was developed into nodule on medium MS + NAA 4 mg / L, MS + CuSO4 2mM + BAP medium 1 mg / L, and MS + CuSO4 2mM + BAP 2 mg / L. Somatic embryogenic callus of accession 032 were able to pass complete globular, heart-shaped, tubular and cotyledonary phase. The most responsive medium for somatic embryogenic callus regeneration, based on the days of the callus phases formation and the number of early-phase cotyledons obtained, were MS medium suplemented with CuSO4 2mM + BAP,and CuSO4 2 mM + BAP 2 mg / L.

Keywords: eggplant, Ralstonia solanacearum, regeneration, cotyledonary, clump, BAP

Katalog: http://perpus.biotek.lipi.go.id/perpus/index.php?p=fstream&fid=3486&bid=16092

Disusun oleh: Ludya AB/Pustakawan

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Cegah Penyebaran Covid-19, LIPI Desinfeksi Kawasan CSC-BG Cibinong

Cibinong, Humas LIPI. Dalam upaya pencegahan penyebaran wabah Covid-19 yang semakin meluas, LIPI melakukan desinfeksi pada fasilitas publik dan semua area satuan kerja di Kawasan Cibinong Science Center-Botanical Garden (CSC-BG) pada Jumat (3/20), yang meliputi P2 Biomaterial, P2 Biologi, P2 Bioteknologi, P2 Limnologi, PPII, Gedung A Kawasan, Gedung Widya Selaras, dan Rusunawa LIPI. “Sesuai arahan Kepala LIPI, untuk upaya pencegahan penyebaran Covid-19 kami melakukan agenda penyemprotan desinfektan di seluruh Kawasan CSC-BG LIPI. Karena area cukup luas yaitu ada sekitar 9 gedung, jadi ada 5 tim yang akan bertugas”, jelas Ahmad Fathoni, Ketua PME Kedeputian Bidang IPH LIPI. 

Fathoni menjelaskan, ada sekitar 100 liter lebih cairan desinfektan yang akan digunakan untuk di seluruh kawasan. Desinfeksi diprioritaskan pada ruangan yang dipakai oleh banyak sivitas LIPI, yaitu ruang rapat, fasilitas gedung dan bagian luar laboratorium (koridor), dan tempat ibadah. “Untuk yang paling krusial seperti Gedung PPII yang masih ada beberapa pertemuan yang diadakan, kedepannya akan dilakukan penyemprotan desinfektan setiap hari, jadi setelah ada pertemuan seperti rapat bisa langsung disterilkan”, ungkapnya.

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